What you'll learn
Advantages of the GC-UV method
Gas chromatography - UV spectrophotometry (GC-UV) is a full scan analytical method, where the compounds eluting from a gas chromatographic column are continuously monitored. The method belongs to the same categories as gas chromatography mass spectroscopy (GC-MS) and gas chromatography Fourier transform IR (GC-FTIR).
Full scan analytical method
Comprehensive qualitative information concerning the identity and chemical class of unknowns in a sample is gained.
High sensitivity
The sensitivities are very high both concerning detection limits, identification limits and classification limits.
UV spectra capabilities
UV-spectra in the vapor phase are very well defined, which is the bases for the identification. The method is particularly suitable for the identification of structural isomers.
UV Spectrometry
Gas Chromatography – GC and GC-UV
Traditional gas chromatography is a well known and very widely used method for chemical detection and analysis installed in hundred thousands of laboratories world wide.
Basically, the heart of the chromatograph is a column, a long tube that separates the various substances by mainly due to their individual boiling points. The separation characteristics depends also upon the chemical nature of the inside surface layer of the column.
The flow of gasses can be detected by various means and a basic gas chromatograph can have various detectors to detect the variations in the out flowing gas. GC-UV is based on the UV light absorption in the gas/vapour phase as the detector.
The wavelength range is extended down into the far-/vacuum-UV. This gives a number of advantages. By carrying out the recording in the vapour phase no spectral shifts, like in the liquid phase, are present and, there are no cut off wavelength limit due to any liquid solvent. At the short UV-wavelength range the majority of the qualitative information can be gained. Moreover, this range also most often shows the highest absorptivities (i. e. highest sensitivities) and many classes of chemical compounds, not considered as UV-absorbing, can be determined with high sensitivity.
Carl Brännhammar
Manager at Labio Scientific®
GC-UV features
From basics to advanced.
Affordable system to analyze gases and liquids.
The GC-UV is a general method of analysis and can be applied within numerous application areas. The results obtained are both qualitative and quantitative. The qualitative results give both identification of individual compounds, including isomers and chemical classification of compounds present in the sample. Classification gives comprehensive information about the chemical class as for example an aliphatic ketone or a substituted benzothiazol and so on. Quantitative determination is based on the knowledge of the light absorbing power of the compound and is often carried out using standards with known concentrations. This is the principle (Lambert Beers law) of spectrophotometry and is considered to be one of the most reliable quantification principle
1. Principles
Lambert Beers law.
2. Detection
Below 150nm.
3. Chemical classes
Selective analysis can be carried out for most of chemical classes.
4. Organic/inorganic
In addition to organic compounds also inorganic can be determined with high sensitivity.
5. Carrier gas
Nitrogen.
6. Software
DataApex Clarity PDA.
7. Instrumental construction
Relatively simple and compact.
8. Sampling
Can be customized depending on application & industry.
Short preview
Sneak peek into several publications
GC-UV has been featured as an analytical method compared to GC-MS and several other techniques. You can discover this on www.labioscientific.com or search on ResearchGate etc.
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GC-UV
For gases or liquids (50% downpayment)
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